Journal
RSC ADVANCES
Volume 12, Issue 6, Pages 3445-3453Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ra08526c
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In this study, a bacterial imprinted polymer-based biosensor was developed for rapid and selective detection of Salmonella typhimurium. The biosensor exhibited high sensitivity and specificity towards the target pathogen and showed no interference signals when tested with other non-targeted pathogens. The biosensor also demonstrated the capability to directly detect the pathogen in minced beef meat samples, eliminating the need for bacterial enrichment steps.
Early detection of pathogens is necessary for food quality monitoring, and increasing the survival rate of individuals. Conventional microbiological methods used to identify microorganisms, starting from bacterial culture and ending with advanced PCR gene identification, are time-consuming, laborious and expensive. Thus, in this study, a bacterial imprinted polymer (BIP)-based biosensor was designed and fabricated for rapid and selective detection of Salmonella typhimurium. Bio-recognition sites were made by creating template-shaped cavities in the electro-polymerized polydopamine matrices on a gold screen-printed electrode. The overall changes of the sensor, during the imprinting process, have been investigated with cyclic voltammetry, atomic force microscopy and scanning electron microscopy. The assay optimization and validation were accomplished, hence the highest sensitivity and selectivity towards S. typhimurium were achieved. As a result, a very low limit of detection of 47 CFU ml(-1), and a limit of quantification of 142 CFU ml(-1) were achieved using the newly-developed biosensor. No interference signals were detected when the S. typhimurium was tested in a mixed culture with other non-targeted pathogens such as Staphylococcus aureus, Listeria monocytogenes and Campylobacter jejuni. Eventually, the biosensor was applied to minced beef meat samples offering not only fast detection but also direct determination with no bacterial enrichment steps.
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