Antibacterial Properties of Biodegradable Silver Nanoparticle Foils Based on Various Strains of Pathogenic Bacteria Isolated from the Oral Cavity of Cats, Dogs and Horses

Frequent occurrence of microbial resistance to biocides makes it necessary to find alternative antimicrobial substances for modern veterinary medicine. The aim of this study was to obtain biodegradable silver nanoparticle-containing (AgNPs) foils synthesized using non-toxic chemicals and evaluation of their activity against bacterial pathogens isolated from oral cavities of cats, dogs and horses. Silver nanoparticle foils were synthesized using sodium alginate, and glucose, maltose and xylose were used as reducing agents. The sizes of AgNPs differed depending on the reducing agent used (xylose < maltose < glucose). Foil without silver nanoparticles was used as control. Bacterial strains were isolated from cats, dogs and horses by swabbing their oral cavities. Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and extended-spectrum beta-lactamase (ESBL) producing E. coli were isolated on selective chromogenic microbiological media. The bactericidal effect of AgNPs foils obtained using non-toxic chemical compounds against E. coli, ESBL, S. aureus and MRSA isolated from oral cavities of selected animals was confirmed in this study. No statistically significant differences were observed between the foils obtained with different reducing agents. Therefore, all types of examined foils proved to be effective against the isolated bacteria.

Automated summary

This study successfully synthesized biodegradable silver nanoparticle-containing foils and confirmed their antibacterial activity against bacterial pathogens isolated from the oral cavities of cats, dogs, and horses. The size of the silver nanoparticles varied depending on the reducing agent used, but all types of examined foils were effective against the isolated bacteria, including E. coli, ESBL, S. aureus, and MRSA.