Journal
MATERIALS
Volume 14, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/ma14237325
Keywords
hyaluronic acid; hydrogels; injectable; tissue engineering; endodontic tissue regeneration
Categories
Funding
- NORTE2020 [NORTE-01-0145-FEDER-000021]
- InjecTE - NFR under NANO2021 [287953]
- FCT [PTDC/NAN-MAT/30595/2017, PTDC/CTM-CTM/29930/2017]
- Secretary of Higher Education, Science, Technology, and Innovation, SENESCYT from Ecuador [CZ06-000132-2017-01]
- Fundação para a Ciência e a Tecnologia [PTDC/CTM-CTM/29930/2017] Funding Source: FCT
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The study investigated an injectable hyaluronic acid-based hydrogel system enriched with platelet lysate for endodontic regeneration. The hydrogels showed suitable working and setting times, a porous microstructure, and supportive properties for cell migration towards dentin surfaces, indicating great clinical potential for endodontic regenerative therapies.
Dental pulp tissue engineering (TE) endeavors to regenerate dentin/pulp complex by combining a suitable supporting matrix, stem cells, and biochemical stimuli. Such procedures foresee a matrix that can be easily introduced into the root canal system (RCS) and tightly adhere to dentin walls to assure the dentin surface's proper colonization with progenitor cells capable of restoring the dentin/pulp complex. Herein was investigated an injectable self-setting hyaluronic acid-based (HA) hydrogel system, formed by aldehyde-modified (a-HA) with hydrazide-modified (ADH), enriched with platelet lysate (PL), for endodontic regeneration. The hydrogels' working (wT) and setting (sT) times, the adhesion to the dentine walls, the hydrogel's microstructure, and the delivery of human dental pulp cells (DPCs) were studied in vitro. Hydrogels incorporating PL showed a suitable wT and sT and a porous microstructure. The tensile tests showed that the breaking point occurs after 4.3106 +/- 1.8677 mm deformation, while in the indentation test after 1.4056 +/- 0.3065 mm deformation. Both breaking points occur in the hydrogel extension. The HA/PL hydrogels exhibited supportive properties and promoted cell migration toward dentin surfaces in vitro. Overall, these results support using PL-laden HA injectable hydrogels (HA/PL) as a biomaterial for DPCs encapsulation, thereby displaying great clinical potential towards endodontic regenerative therapies.
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