Journal
MATERIALS
Volume 14, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/ma14216600
Keywords
genipin; crosslinking; hydrogel; aldehyde; cytotoxicity; IC50; injectable
Categories
Funding
- JSPS KAKENHI [15K01321]
- Grants-in-Aid for Scientific Research [15K01321] Funding Source: KAKEN
Ask authors/readers for more resources
The study found that the crosslinking rate of warmed genipin (w-GE) was higher than intact genipin (n-GE), but the degree of crosslinking of n-GE gels was higher at 12 hours. Additionally, the ISO 10993-5 standard method showed that the equivalent IC50 values for w-GE and n-GE were 0.173 mM and 0.166 mM, respectively. Furthermore, custom-made cytotoxicity tests indicated that the acute cytotoxicity of w-GE was significantly higher than n-GE at concentrations between 0.1-5 mM.
The aim of the present study was to compare the acute and cumulative cytotoxicity of intact (n-GE) and warmed genipin (w-GE), while investigating the differences in crosslinking capabilities of these two genipins by rheological and mechanical tests. The n-GE solution was prepared by dissolving genipin powder in a sodium phosphate buffer solution. The w-GE solution was prepared by warming the n-GE solution at 37 & DEG;C for 24 h. The mechanical tests for chitosan (CH)/genipin gels showed the crosslinking rate of w-GE was much greater than that of n-GE up until 6 h after preparation, whereas the degree of crosslinking of CH/n-GE gels became higher at 12 h. The ISO 10993-5 standard method, which is established specifically for evaluating cumulative cytotoxicity, determined equivalent IC50 for w-GE (0.173 mM) and n-GE (0.166 mM). On the other hand, custom-made cytotoxicity tests using a WST-8 assay after 1 h of cultivation showed that the acute cytotoxicity of w-GE was significantly higher than that of n-GE at concentrations between 0.1-5 mM. The acute cytotoxicity of w-GE should be taken into consideration in its practical uses, despite the fact that the much faster crosslinking of w-GE is useful as an effective cross linker for in-situ forming gels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available