Journal
CONSERVATION GENETICS RESOURCES
Volume 14, Issue 1, Pages 41-45Publisher
SPRINGER
DOI: 10.1007/s12686-021-01248-x
Keywords
Tea oil; Resource conservation; SSR; Dendrogram
Categories
Funding
- Education Department of Guangdong Province [2020GCZX009, 2019KZDZX2035]
- Research on Key Technology and Product Development of Camellia Oil Rich in Functional Active Ingredients [2017KJCX005]
- Applied Technology and Collaborative Innovation Center of Guangdong Agriculture Industry Business Polytechnic [XJZX1902]
- Global Visiting Fellowship, BrainKorea 21 Four, Seoul National University, Seoul, Korea
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SSRs derived from EST sequences in this study can be used for resource identification and dendrogram construction for resource collection and conservation.
Tea oil plants are economically valuable as a chemical feedstock, a bioenergy source, and a dietary supplement. The lack of molecular markers for genetic resource clustering for conservation restricts research on these species. Based on EST sequences, 28,120 SSR primer pairs were found in this study. Of those SSRs, 192 had a successful amplification rate of 93.23% in Camellia oleifera and high transferability rates in C. semiserrata (77.09%), C. gauchowensis (84.92%), C. japonica (78.77%), and C. polyodonta (89.39%). The genetic distance (GD) between 54 accessions, as determined by sixteen SSRs, ranged from 0.26 to 2.26 (GD > 0), indicating that these accessions could be discriminated completely by these sixteen SSRs. We suggest that the EST-SSRs generated in this study will be useful for resource identification and dendrogram construction for resource collection and conservation.
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