Journal
BIOMEDICAL OPTICS EXPRESS
Volume 13, Issue 3, Pages 1173-1187Publisher
Optica Publishing Group
DOI: 10.1364/BOE.445945
Keywords
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Funding
- Horizon 2020 Framework Programme [964481]
- Universita degli Studi di Milano-Bicocca [2018-ATE-0070, 2019-ATE-0126]
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This study proposes a non-destructive photo-thermal imaging method that can characterize the distribution and concentration of melanin pigments in excised melanoma biopsies. It provides a label-free super-resolution far-infrared thermal imaging of tissue sections, allowing for the quantitative analysis of melanin content. The proposed method could complement traditional histopathology in the characterization of pigmented lesions ex-vivo.
Surgical excision followed by histopathological examination is the gold standard for melanoma screening. However, the color-based inspection of hematoxylin-and-eosin-stained biopsies does not provide a space-resolved quantification of the melanin content in melanocytic lesions. We propose a non-destructive photo-thermal imaging method capable of characterizing the microscopic distribution and absolute concentration of melanin pigments in excised melanoma biopsies. By exploiting the photo-thermal effect primed by melanin absorption of visible laser light we obtain label-free super-resolution far-infrared thermal images of tissue sections where melanin is spatially mapped at sub-diffraction 40-mu m resolution. Based on the finite-element simulation of the full 3D heat transfer model, we are able to convert temperature maps into quantitative images of the melanin molar concentration on B16 murine melanoma biopsies, with 4.10(-4) M concentration sensitivity. Being readily applicable to human melanoma biopsies in combination with hematoxylin-and-eosin staining, the proposed approach could complement traditional histopathology in the characterization of pigmented lesions ex-vivo. (C) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
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