Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-26879-4
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Funding
- Knut and Alice Wallenberg Foundation [KAW 2014.071, 2015.0063]
- Uppsala County Council
- Swedish Foundation for Strategic Research
- Swedish Government, StemTherapy
- HSE University Basic Research Program
- Russian Science Foundation [17-14-01338]
- RuFu
- Novo Nordisk A/S
- SRP Diabetes
- Swedish Research Council [2020-00687]
- Family Erling-Persson Foundation
- Swedish Society of Medicine (Stiftelsen Albert Nilssons forskningsfond) [SLS-961262, 1086]
- Ministry of Science and Higher Education of the Russian Federation [075-15-2020-899]
- Swedish Research Council [2020-00687] Funding Source: Swedish Research Council
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The authors developed a method to simultaneously measure protein expression and thermal stability changes during cell type transitions, and applied this approach to study differences between human pluripotent stem cells and other cell types. They detected alterations of protein properties in numerous cellular pathways and components, proposing a method for maintaining pluripotency.
To characterize molecular changes during cell type transitions, the authors develop a method to simultaneously measure protein expression and thermal stability changes. They apply this approach to study differences between human pluripotent stem cells, their progenies, parental and allogeneic cells. Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro.
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