Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-26736-4
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Funding
- McKnight Foundation
- Human Frontier Science Program [RGP0027/2016]
- NSF [1934288, 1450824]
- NIH [NINDS R01NS091335, NEI R01EY024294]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1450824] Funding Source: National Science Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1934288] Funding Source: National Science Foundation
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The Diesel2p system is an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging, offering subcellular resolution over a large field-of-view. The system demonstrates flexibility and various use cases for calcium imaging of neurons in the living brain.
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of similar to 25 mm(2), encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.
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