Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 46, Issue 5-6, Pages 323-332Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2016.01.001
Keywords
FIKK kinase; Toxoplasma; Intracellular pathogen; Parasite; Serine threonine kinases; Basal complex
Categories
Funding
- National Institutes of Health, USA [AI072028, AI107431, AI108006, AI081924, AI110690]
- Deutsche Forschungsgemeinschaft, Germany
- Qatar National Research Fund, Qatar [JSREP: 05-007-3-002]
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FIKK kinases are a novel family of kinases unique to the Apicomplexa. While most apicomplexans encode a single FIKK kinase, Plasmodium falciparum expresses 21 and piroplasms do not encode a FIKK kinase. FIKK kinases share a conserved C-terminal catalytic domain, but the N-terminal region is highly variable and contains no known functional domains. To date, FIKK kinases have been primarily studied in P. falciparum and Plasmodium berghei. Those that have been studied are exported from the parasite and associate with diverse locations in the infected erythrocyte cytosol or membrane. Deletion of individual P. falciparum FIKK kinases indicates that they may play a role in modification of the infected erythrocyte. The current study characterises the single FLICK gene in Toxoplasma gondii to evaluate the importance of the FIKK kinase in an apicomplexan that has a single FIKK kinase. The TgFIKK gene encoded a protein of approximately 280 kDa. Endogenous tagging of the FIKK protein with Yellow Fluorescent Protein showed that the FIKK protein exclusively localised to the posterior end of tachyzoites. A Yellow Fluorescent Protein-tagged FIKK and a Ty-tagged FIKK both co-localised with T. gondii membrane occupation and recognition nexus protein to the basal complex and were localised apical to inner membrane complex protein-5 and Centring. Deletion of TgFIKK, surprisingly, had no detectable effect on the parasite's lytic cycle in vitro in human fibroblast cells or in acute virulence in vivo. Thus, our results clearly show that while the FIKK kinase is expressed in tachyzoites, it is not essential for the lytic cycle of T. gondii. (C) 2016 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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