Journal
MBIO
Volume 12, Issue 5, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/mBio.02408-21
Keywords
HIV-1; dendritic cell; complement; CR4; type I IFN; antiviral immunity; cytosolic sensor; complement receptors; dendritic cells; human immunodeficiency virus
Categories
Funding
- EC
- Bill and Melinda Gates GHRC-CAVD Project
- Austrian National Bank (OeNB Jubilaumsfonds) [17614]
- Austrian Science Fund (FWF) [P33510]
- graduate program MCBO [W11]
- graduate program CBD (Cellular Basis of Diseases) [FWF DOC 82]
- Austrian Science Fund (FWF) [P33510] Funding Source: Austrian Science Fund (FWF)
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Complement-opsonized HIV-1 triggers efficient antiviral responses in dendritic cells by activating multiple signaling pathways. Complement plays a crucial role in inducing effective antiviral immune responses by preventing HIV-1 suppressive mechanisms and activating specific cytosolic sensors.
Complement-opsonized HIV-1 triggers efficient antiviral type I interferon (IFN) responses in dendritic cells (DCs), which play an important role in protective responses at the earliest stages in retroviral infection. In contrast, HIV-1 suppresses or escapes sensing by STING-and MAVS-associated sensors. Here, we identified a complement receptor-mediated sensing pathway, where DCs are activated in CCR5/RLR (RIG-I/MDA5)/MAVS/TBK1-dependent fashion. Increased fusion of complementopsonized HIV-1 via complement receptor 4 and CCR5 leads to increased incoming HIV-1 RNA in the cytoplasm, sensed by a nonredundant cooperative effect of RIG-I and MDA5. Moreover, complement-opsonized HIV-1 down-modulated the MAVS-suppressive Raf-1/PLK1 pathway, thereby opening the antiviral recognition pathway via MAVS. This in turn was followed by MAVS aggregation and subsequent TBK1/IRF3/NF-kappa B activation in DCs exposed to complementbut not non-opsonized HIV-1. Our data strongly suggest that complement is important in the induction of efficient antiviral immune responses by preventing HIV-1 suppressive mechanisms as well as inducing specific cytosolic sensors. IMPORTANCE Importantly, our study highlights an unusual target on DCs-the a chain of complement receptor 4 (CR4) (CD11c)-for therapeutic interventions in HIV 1 treatment. Targeting CD11c on DCs mediated a potent antiviral immune response via clustering of CR4 and CCR5 and subsequent opening of an antiviral recognition pathway in DCs via MAVS. This novel finding might provide novel tools for specifically boosting endogenous antiviral immunity via CR4, abundantly expressed on multiple DC subsets.
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