Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 89, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jff.2022.104966
Keywords
Acute lung injury; Phytosterols; LXRs; RAW264.7
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Funding
- Yihaikerry Nutrition and Food Safety Foundation, Chinese Nutrition Society [CNS-W2018A45]
- Project of Technology Innovation and Application, Chongqing, China [cstc2019jscx-msxmX0280]
- Postgraduate Innovation Project of Chongqing Municipal Education Commission, Chongqing, China [CYS20208]
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The study found that phytosterols can partially alleviate pulmonary inflammation in LPS-induced acute lung injury (ALI) by activating the LXRs/ABCA1 pathway.
Phytosterols (PS) showed anti-inflammatory activities in acute lung injury (ALI), but the mechanism needs to be further elucidated. In this study, male C57BL/6J mice were gavaged with PS (75 mg/kg or 150 mg/kg) for 7 consecutive days, mice ALI were induced by tracheal instillation of LPS (50 mu L, 1 mg/mL). In vitro, RAW264.7 cells were incubated with LPS in the presence or absence of 50 mu M beta-sitosterol. Enzyme-linked immunosorbent assay was used to detect levels of inflammatory cytokines. Real-time quantitative PCR and western blot analysis were used to detect changes in nuclear factor-kB (NF-kappa B) and liver X receptors (LXRs) pathways. The results showed that PS administration activated LXRs/ABCA1 pathway and significantly ameliorated LPS-induced pulmonary inflammation of ALI. The role of phytosterols was verified in LPS-stimulated RAW264.7 cells and the protective effect was weakened by administration of LXR antagonist GSK2033. These findings suggested that PS ameliorates LPS-induced ALI partially by activating the LXRs/ABCA1 pathway.
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