Journal
VIRUSES-BASEL
Volume 14, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/v14020329
Keywords
SARS-CoV-2; vaccines; CD8(+) T cell immunity; extracellular vesicles; Nef
Categories
Funding
- Istituto Superiore di Sanita, Rome, Italy [R220]
- [NR-52284]
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Engineered extracellular vesicles can induce a potent SARS-CoV-2 CD8(+) T cell immune response and generate long-lasting lung-specific immune memory. This immunization approach is significant in addressing the limitations of current anti-SARS-CoV-2 vaccines.
SARS-CoV-2-specific CD8(+) T cell immunity is expected to counteract viral variants in both efficient and durable ways. We recently described a way to induce a potent SARS-CoV-2 CD8(+) T immune response through the generation of engineered extracellular vesicles (EVs) emerging from muscle cells. This method relies on intramuscular injection of DNA vectors expressing different SARS-CoV-2 antigens fused at their N-terminus with the Nef(mut) protein, i.e., a very efficient EV-anchoring protein. However, quality, tissue distribution, and efficacy of these SARS-CoV-2-specific CD8(+) T cells remained uninvestigated. To fill the gaps, antigen-specific CD8(+) T lymphocytes induced by the immunization through the Nef(mut)-based method were characterized in terms of their polyfunctionality and localization at lung airways, i.e., the primary targets of SARS-CoV-2 infection. We found that injection of vectors expressing Nef(mut)/S1 and Nef(mut)/N generated polyfunctional CD8(+) T lymphocytes in both spleens and bronchoalveolar lavage fluids (BALFs). When immunized mice were infected with 4.4 lethal doses of 50% of SARS-CoV-2, all S1-immunized mice succumbed, whereas those developing the highest percentages of N-specific CD8(+) T lymphocytes resisted the lethal challenge. We also provide evidence that the N-specific immunization coupled with the development of antigen-specific CD8(+) T-resident memory cells in lungs, supporting the idea that the Nef(mut)-based immunization can confer a long-lasting, lung-specific immune memory. In view of the limitations of current anti-SARS-CoV-2 vaccines in terms of antibody waning and efficiency against variants, our CD8(+) T cell-based platform could be considered for a new combination prophylactic strategy.
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