Journal
VIRUSES-BASEL
Volume 13, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/v13122474
Keywords
COVID-19; RT-PCR; N gene; GeneFinder; mutation
Categories
Funding
- Butantan Institute
- Sao Paulo Research Foundation (FAPESP) [2020/10127-1, 2020/05367-3, 2013/08135-2]
- Central Public Health Laboratories, Blood Center of Ribeirao Preto and supported by the Brazilian Ministry of Health and the Pan American Health Organization PAHO/WHO [APO21-00010098]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401119/2020-3]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
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The article highlights the evolution of the COVID-19 virus and the potential impact on RT-PCR testing accuracy, identifying gene mutations that affect N gene detection and emphasizing the importance of continuous monitoring of genetic mutations to ensure diagnostic effectiveness.
The current COVID-19 pandemic demands massive testing by Real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction), which is considered the gold standard diagnostic test for the detection of the SARS-CoV-2 virus. However, the virus continues to evolve with mutations that lead to phenotypic alterations as higher transmissibility, pathogenicity or vaccine evasion. Another big issue are mutations in the annealing sites of primers and probes of RT-PCR diagnostic kits leading to false-negative results. Therefore, here we identify mutations in the N (Nucleocapsid) gene that affects the use of the GeneFinder COVID-19 Plus RealAmp Kit. We sequenced SARS-CoV-2 genomes from 17 positive samples with no N gene detection but with RDRP (RNA-dependent RNA polymerase) and E (Envelope) genes detection, and observed a set of three different mutations affecting the N detection: a deletion of 18 nucleotides (Del28877-28894), a substitution of GGG to AAC (28881-28883) and a frameshift mutation caused by deletion (Del28877-28878). The last one cause a deletion of six AAs (amino acids) located in the central intrinsic disorder region at protein level. We also found this mutation in 99 of the 14,346 sequenced samples by the Sao Paulo state Network for Pandemic Alert of Emerging SARS-CoV-2 variants, demonstrating the circulation of the mutation in Sao Paulo, Brazil. Continuous monitoring and characterization of mutations affecting the annealing sites of primers and probes by genomic surveillance programs are necessary to maintain the effectiveness of the diagnosis of COVID-19.
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