Journal
VIRUSES-BASEL
Volume 13, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/v13102025
Keywords
fruit tree virus; Prune dwarf virus; ilarvirus; infectious clone; next generation sequencing
Categories
Funding
- Agriculture and Agri-Food Canada [J-002199]
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2020-06416]
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This study identified PDV from sweet cherry in the Niagara fruit belt of Ontario, Canada, constructed an infectious cDNA clone inducing dwarfing phenotype in cherry, and found that the clone was infectious on cucurbits and Nicotiana benthamiana but not on Arabidopsis thaliana.
Prune dwarf virus (PDV) is a member of ilarviruses that infects stone fruit species such as cherry, plum and peach, and ornamentally grown trees worldwide. The virus lacks an RNA silencing suppressor. Infection by PDV either alone, or its mixed infection with other viruses causes deteriorated fruit marketability and reduced fruit yields. Here, we report the molecular identification of PDV from sweet cherry in the prominent fruit growing region of Ontario, Canada known as the Niagara fruit belt using next generation sequencing of small interfering RNAs (siRNAs). We assessed its incidence in an experimental farm and determined the full genome sequence of this PDV isolate. We further constructed an infectious cDNA clone. Inoculation of the natural host cherry with this clone induced a dwarfing phenotype. We also examined its infectivity on several common experimental hosts. We found that it was infectious on cucurbits (cucumber and squash) with clear symptoms and Nicotiana benthamiana without causing noticeable symptoms, and it was unable to infect Arabidopsis thaliana. As generating infectious clones for woody plants is very challenging with limited success, the PDV infectious clone developed from this study will be a useful tool to facilitate molecular studies on PDV and related Prunus-infecting viruses.
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