Journal
VIRUSES-BASEL
Volume 14, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/v14020190
Keywords
Listeria monocytogenes; phage therapy; gut microbiome
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Funding
- Intralytix, Inc.
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In this study, the effect of a bacteriophage cocktail (tentatively designated as the Foodborne Outbreak Pill (FOP)) on Listeria monocytogenes levels in simulated gastrointestinal models was investigated. The FOP was found to be robust in inhibiting the growth of L. monocytogenes, similar to antibiotic treatment, without affecting the commensal bacteria. Furthermore, the FOP showed better efficacy in protecting Caco-2 cells from invasion by L. monocytogenes compared to the antibiotic.
In this study, we examined the effect of a bacteriophage cocktail (tentatively designated as the Foodborne Outbreak Pill (FOP)) on the levels of Listeria monocytogenes in simulated small intestine, large intestine, and Caco-2 model systems. We found that FOP survival during simulated passage of the upper gastrointestinal was dependent on stomach pH, and that FOP robustly inhibited L. monocytogenes levels with effectiveness comparable to antibiotic treatment (ampicillin) under simulated ilium and colon conditions. The FOP did not inhibit the commensal bacteria, whereas ampicillin treatment led to dysbiosis-like conditions. The FOP was also more effective than an antibiotic in protecting Caco-2 cells from adhesion and invasion by L. monocytogenes (5-log reduction vs. 1-log reduction) while not triggering an inflammatory response. Our data suggested that the FOP may provide a robust protection against L. monocytogenes should the bacterium enter the human gastrointestinal tract (e.g., by consumption of contaminated food), without deleterious impact on the commensal bacteria.
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