4.4 Article

Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System

Journal

ZEBRAFISH
Volume 19, Issue 1, Pages 32-35

Publisher

MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2021.0072

Keywords

embryo; zebrafish; array; behavior; ecotoxicology; neurotoxicity; automated; screening

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This study demonstrates the advantages of using custom embryo arrays and an ultra-high-definition video imaging system for large-scale chemobehavioral phenotyping with zebrafish embryos. It also presents a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of embryos.
Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack of effective embryo in-test positioning, wide-field imaging, and high-throughput bioinformatic analytics. In this study, we demonstrate advantages of using custom large-density embryo arrays in conjunction with an open-source ultra-high-definition video imaging system. Moreover, we present a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of specimens in photomotor response bioassays. The system validation was showcased in a proof-of-concept neurotoxicity analysis.

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