Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 38, Issue 4, Pages -Publisher
SPRINGER
DOI: 10.1007/s11274-021-03115-1
Keywords
High gravity brewing; Lager yeast; Metabolomic analysis; Tolerance mechanism; Transcriptomic analysis
Categories
Funding
- National Science Foundation [31571942, 31771963, 439, 31601558]
- Program of Introducing Talents of Discipline to Universities [111-2-06]
- 440 national first-class discipline program of Light Industry Technology and Engineering [LITE2018-441 13]
- Collaborative Innovation Center of 442 Jiangsu Modern Industrial Fermentation from Jiangnan University
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Through transcriptomic and metabolomic analysis, genes related to stress tolerance in high gravity brewing were screened, and their functions were verified through overexpression and knockdown strains. The findings help reveal the stress tolerance mechanism in high gravity brewing and improve yeast performance.
The fermentation performance of yeast is the key of beer production. High gravity brewing is a commonly used technique in industrial lager beer production and it is environmentally friendly. Therefore, there has been extensive effort toward improving high gravity brewing. In this study, through transcriptomic and metabolomic analysis of two homologous lager yeasts, genes that relate to stress tolerance in high gravity brewing were screened. The results showed EMP pathway and multiple amino acid metabolism pathway were the most enriched pathways, and pyruvate might be the core metabolite. Overexpression and knockdown strains were constructed to verify the genes' functions. The overexpression of MAN2, PCL1 and PFK26 genes were beneficial to fermentation without significantly changes in flavor profiles. The relative intracellular ATP levels can help us understand the change of metabolic flux such as enhancement of sugar consumption. This work is helpful to reveal the stress tolerance mechanism of high gravity brewing and breed yeast strains with improved performance.
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