Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 34, Issue -, Pages 259-262Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2016.03.015
Keywords
emu-miR-71; Macrophage; Exosome
Categories
Funding
- Key Laboratory of parasite and Vector Biology, MOH, China [WSBKTKT201301]
- Natural Science Foundation of Gansu Province [1308RJZA105]
- National Natural Science Foundation of China [81371841, 81371842]
- National Key Basic Research Program (973 program) of China [2015CB150300]
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The microRNAs (miRNAs) are a class of small regulatory non-coding RNA that contributes to the activation of host-pathogen cross-talk during infection. In helminthes, miR-71 is highly conserved and it has recently been detected in nematode exosomes, as well as in the sera and/or fluids of infected humans and mice. However, the role of miR-71 during infection remains poorly characterized. Herein, we show that Ago1 and Ago4, which encode key components of the small RNA-induced silencing complex (RISC), were up-regulated in murine macrophage RAW264.7 cells transfected by Echinococcus multilocularis miR-71 (emu-miR-71) mimics. Using a miRNA PCR array, none of the 84 miRNAs involved in inflammation or autoimmunity were significantly up- or down regulated in the transfected cells (p > 0.05). Although it did not influence IL-10 production by the treated cells (p > 0.05), the mimics significantly repressed the production of NO 12 h after treatment with LPS and IFN-gamma (p < 0.01), identifying another potential mechanism whereby parasites can carefully regulate host levels of NO. These findings indicate that the release of parasite-derived miR-71 into hosts can affect the functions of macrophages, and possibly represents an exciting direction for studies of the interplay between parasites and hosts. (C) 2016 Elsevier B.V. All rights reserved.
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