Interleukin-37 suppresses ICAM-1 expression in parallel with NF-κB down-regulation following TLR2 activation of human coronary artery endothelial cells

Introduction: The inflammatory receptor Toll-like receptors (TLRs) activation could induce endothelial inflammatory responses, which plays an important role in the development of many diseases including atherosclerosis. We already found that TLR2 activation of Peptidoglycan (PGN) stimulation could increase intercellular adhesion molecule-1 (ICAM-1) expression in HCAEC5. Since anti-inflammatory cytokine interleukin (1)-37 exhibits intra- and extracellular properties for suppressing innate inflammation, we want to investigate whether IL-37 suppresses ICAM-1 expression and this effect is in parallel with the inhibition of nuclear factor kappa B (NF-kappa B) activation upon PGN stimulation in HCAECs. Methods: HCAECs were treated with IL-37-transfection plasmid or silent mRNA or nothing for 24 h, and we test IL-37 expression by immunoblotting. Same treatments prior to PGN stimulation (10 mu g/ml), we analyzed the expression of ICAM-1 and NF-kappa B mRNA at 0, 30 min, 1 and 2 h by real-time PCR. ICAM-1 protein at 24 h and NF-kappa B activation at 0-2 h were measured by immunoblotting. Results: IL-37 and silent IL-37 transfection change the expression of IL-37 protein. Stimulation of PGN increased both NF-kappa B activation and ICAM-1 expression at mRNA and protein level, but these inflammatory cytokines' expression was significantly decreased in IL-37-transfection cells. Interestingly, both NF-kappa B activation and ICAM-1 expression were significantly increased when IL-37 was silent. Conclusions: As an anti-inflammatory cytokine, IL-37 could decrease both NF-kappa B and ICAM-1 expression upon TLR2 activation in HCAEC5. The suppressed effect of IL-37 on ICAM-1 may be due to its inhibition on NF-kappa B. (C) 2016 The Authors. Published by Elsevier B.V.