Bioremediation of trichloroethylene-polluted groundwater using emulsified castor oil for slow carbon release and acidification control

In this study, the emulsified castor oil (ECO) substrate was developed for a long-term supplement of biodegradable carbon with pH buffering capacity to anaerobically bioremediate trichloroethylene (TCE)-polluted groundwater. The ECO was produced by mixing castor oil, surfactants (sapindales and soya lecithin [SL]), vitamin complex, and a citrate/sodium phosphate dibasic buffer system together for slow carbon release. Results of the emulsification experiments and microcosm tests indicate that ECO emulsion had uniform small droplets (diameter = 539 nm) with stable oil-in-water characteristics. ECO had a long-lasting, dispersive, negative zeta potential (-13 mv), and biodegradable properties (viscosity = 357 cp). Approximately 97% of TCE could be removed with ECO supplement after a 95-day operational period without the accumulation of TCE dechlorination byproducts (dichloroethylene and vinyl chloride). The buffer system could neutralize acidified groundwater, and citrate could be served as a primary substrate. ECO addition caused an abrupt TCE adsorption at the initial stage and the subsequent removal of adsorbed TCE. Results from the next generation sequences and real-time polymerase chain reaction (PCR) indicate that the increased microbial communities and TCE-degrading bacterial consortia were observed after ECO addition. ECO could be used as a pH-control and carbon substrate to enhance anaerobic TCE biodegradation effectively. Practitioner Points Emulsified castor oil (ECO) contains castor oil, surfactants, and buffer for a slow carbon release and pH control. ECO can be a long-term carbon source for trichloroethylene (TCE) dechlorination without causing acidification. Y TCE removal after ECO addition is due to adsorption and reductive dechlorination mechanisms.

Automated summary

Emulsified castor oil (ECO) was developed as a substrate for long-term supplement of biodegradable carbon with pH buffering capacity to effectively remediate trichloroethylene (TCE) contaminated groundwater. The ECO emulsion had uniform droplets, negative zeta potential, and biodegradable properties facilitating the removal of TCE without accumulation of dechlorination byproducts. Addition of ECO led to an increase in microbial communities and TCE-degrading bacterial consortia, enhancing anaerobic TCE biodegradation.