Journal
VIRUS RESEARCH
Volume 304, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.virusres.2021.198549
Keywords
Enterovirus D68; 3D polymerase; PGAM5; IEN-beta; Mitochondria; Innate immunity; RIG-I pathway
Categories
Funding
- CAMS Innovation Fund for Medical Sciences [2016-I2M-1-014]
- Basic Research Project of Science and Technology of Yunnan Province [202001AT070143]
- Basic Scientific Research Funding of National Colleges and Universities [3332020066]
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During EV-D68 replication, the interaction between 3D(Poly) and PGAM5 affects mitochondrial dynamics and inhibits the expression of IFN-beta by impacting the RIG-I-like receptor signal pathway.
Enterovirus D68 (EV-D68) belongs to the Picornaviridae family and can lead to severe clinical manifestations in the respiratory system. The 3D-polymerase (3D(Poly)) is an important nonstructural protein during EV-D68 replication, but few studies have addressed its interaction with the host antiviral response during EV-D68 infection. Here, we used human bronchial epithelial cells to investigate the impact of the 3D(Poly) on the mitochondrial dynamics and innate immune response. The results showed that the number and morphology of the mitochondria in 16HBE cells was affected during the early stage of infection, and these effects included the cellular apoptosis. Moreover, we found that the 3D(Poly) of EV-D68 can interact with PGAM5 and promote mitofusin 2 protein upregulation, and subsequently, 3D(Poly) impairs IFN-beta expression by impacting the activation of the RIG-I receptor signaling pathway. Our findings suggest that during EV-D68 replication, the 3D(Poly), via its interaction with PGAM5, can affect the mitochondrial dynamics and suppress the expression of IFN-beta by impacting the RIG-I-like receptor signal pathway.
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