4.2 Article

Aqueous angiography in pre-glaucomatous and glaucomatous ADAMTS10-mutant canine eyes: A pilot study

Journal

VETERINARY OPHTHALMOLOGY
Volume 25, Issue -, Pages 72-83

Publisher

WILEY
DOI: 10.1111/vop.12938

Keywords

ADAMTS10; aqueous angiography; canine; conventional outflow; indocyanine green; open-angle glaucoma

Funding

  1. Michigan State University College of Veterinary Medicine Endowed Research Funds
  2. Michigan State University startup funds
  3. NIH [R01-EY025752, R01EY030501]
  4. Bright Focus Foundation
  5. Research to Prevent Blindness

Ask authors/readers for more resources

Evaluation of intravenous scleral and intracameral aqueous angiography in normal-tension and high-tension glaucomatous canine eyes showed distinct patterns of vascular flow, with a higher prevalence of laminar flow observed in hypertensive eyes.
Objective To evaluate intravenous scleral and intracameral aqueous angiography in normotensive (n = 4) and hypertensive glaucomatous (n = 6) ADAMTS10-mutant canine eyes. Animals studied Ten ADAMTS10-mutant dogs were used in this study. Procedures Dogs were sedated and one eye from each dog underwent scleral angiography following intravenous injection of 0.25% indocyanine green (ICG). After a 24-h recovery period, the same eye underwent aqueous angiography via intracameral administration of ICG. Imaging of identical scleral sectors from the same eye was performed using a Heidelberg Spectralis(R) Confocal Scanning Laser Ophthalmoscope. Intrascleral vessel depth and lumen diameters were measured using Heidelberg Spectralis(R) optical coherence tomography and computer software. Results Scleral angiography permitted visualization of vascular components associated with conventional aqueous humor outflow pathways with an average time from injection to fluorescence of 35.8 +/- 10.6 s (mean +/- SD). Two normotensive eyes (2/10;20%) demonstrated turbulent dye movement, while 4 hypertensive eyes (4/10;40%) exhibited laminar flow. Aqueous angiography demonstrated dye fluorescence within the post-trabecular conventional aqueous humor outflow pathways in all 10 eyes at 34.3 +/- 11.0 s post-injection. Sectoral and dynamic outflow patterns were observed primarily within the superotemporal sector in nine eyes (9/10; 90%). Seven eyes (7/10; 70%) demonstrated pulsatile dye movement and five eyes (5/10; 50%) exhibited laminar flow. The degree of laminar movement of dye was greatest in hypertensive eyes. Vessel lumen diameters measured 133.85 +/- 28.36 mu m and 161.18 +/- 6.02 mu m in hypertensive and normotensive eyes, respectively. Conclusions Aqueous angiography allowed for visualization of fluorescent dye in the superotemporal sclera. Laminar flow and smaller lumen vessels were observed mainly in hypertensive eyes.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.2
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available