Journal
ULTRAMICROSCOPY
Volume 230, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.ultramic.2021.113376
Keywords
Transmission electron microscopy; TEM; Electron diffraction; Holography; Electron holography; Twin image; Nano-crystals; Bacteriorhodopsin; Structural biology; Protein structure
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Funding
- OIST Graduate University
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Crystal diffraction is a well-established technique for high-resolution structural analysis, but it can smear out imperfections and defects in the crystal. Bragg holography offers a way to image nano-crystals at a defocus distance, allowing separation of diffracted beams without turning them into peaks. This technique provides a complete complex-valued wavefront with information about atomic distribution, including defects, and has been successfully demonstrated for gold nano-crystals with feasibility shown for biological nano-crystals.
Crystal diffraction is a well-established technique for high-resolution structural analysis of material science and biological samples. However, the recovered structure is a result of averaging over all the unit cells in the crystal, which smears out the imperfections, atomic defects, or asymmetries and chiral properties of the individual molecules. We propose Bragg holography, where a nano-crystal is imaged at a defocus distance allowing separation of the diffracted beams, without turning them into peaks. The presence of a reference wave gives rise to a Bragg hologram, which can be reconstructed by conventional holographic reconstruction algorithms. The recovered complex-valued wavefront contains the complete information about the atomic distribution in the crystal, including defects. Bragg holography is demonstrated for gold nano-crystals, and its feasibility for biological nano-crystals is shown.
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