Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi

In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L-1 laccase cocktail removed 50% Diclofenac, 97% beta-Naphthol and 71% 2,4 Dichlorophenol within 8 h of reaction and 78% for 5,7-Diiodo-8-hydroxyquinoline within 3.5 h; at initial concentrations of 10 mg L-1 and at 25 degrees C. Furthermore, this enzyme cocktail also removed in excess of 53% all tested compounds in a real groundwater sample from northwestern Mexico. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).