Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 143, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2021.116377
Keywords
Rapid PCR; Thermal cycling; Pathogen detection; COVID-19; Microfluidics; Point-of-care testing (POCT)
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Funding
- National Natural Science Foundation of China [81871505, 61971026]
- Fundamental Research Funds for the Central Universities [XK1802-4]
- National Science and Technology Major Project [2018ZX10732101-001-009]
- technological innovation team from Beijing University of Chemical Technology [buctylkjcx06]
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This review focuses on the latest rapid microfluidic PCR technology, summarizing and discussing the reactor and thermal cycling implementation in PCR. The balance between speed and sensitivity in microfluidic PCR is explored.
PCR has been widely used in different fields including molecular biology, pathogen detection, medical diagnosis, food detection and etc. However, the difficulty of promoting PCR in on-site point-of-care testing reflects on challenges relative to its speed, convenience, complexity, and even cost. With the emerging state-of-art of microfluidics, rapid PCR can be achieved with more flexible ways in microreactors. PCR plays a critical role in the detection of SARS-CoV-2. Under this special background of COVID-19 pandemic, this review focuses on the latest rapid microfluidic PCR. Rapid PCR is concluded in two main features, including the reactor (type, size, material) and the implementation of thermal cycling. Especially, the compromise between speed and sensitivity with microfluidic PCR is explored based on the system ratio of (thermal cycling time)/(reactor size). Representative applications about the detection of pathogens and SARS-CoV-2 viruses based on rapid PCR or other isothermal amplification are discussed as well. (C) 2021 Elsevier B.V. All rights reserved.
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