Synergistic toxicity of some cyanobacterial oligopeptides to physiological activities of Daphnia magna (Crustacea)

Anabaenopeptins and microcystins are oligopeptides produced by bloom-forming cyanobacteria. We determined in vivo effects of anabaenopeptin-B (AN-B) and two variants of microcystins of different hydrophobicity (MC-LR and MC-LF) on the physiology of Daphnia magna. Heart rate, thoracic limb activity and post-abdominal claw activity were determined by digital video analysis and oxygen consumption by Oxygraph + system. EC50 calculation and isobole methodology for interactive effects of AN-B and MC-LR mixture were used.Daphnids' responses to all three oligopeptides were concentration- and time-dependent. MC-LF was the most potent inhibitor of heart rate, thoracic limb activity, post-abdominal claw activity and oxygen consumption. ANB was more toxic than MC-LR toward oxygen consumption; it inhibited the movements of limbs and postabdominal claw similarly to MC-LR, but did not inhibit heart rate. The strongest toxic effects were induced by the binary mixture of AN-B with MC-LR at the sum concentration equal to the concentration of the single compounds. First time direct synergistic toxic effects of the cyanopeptides on all the physiological parameters were found. The obtained results explain stronger disturbances in aquatic organisms caused by cyanobacterial cell contents than the individual cyanopeptides present even at higher concentrations. Other metabolites and their interactions need further studies.

Automated summary

This study investigated the effects of anabaenopeptins and microcystins on the physiology of Daphnia magna. The results showed that MC-LF was the most potent inhibitor, while AN-B and MC-LR had varying degrees of inhibition on different physiological parameters. The binary mixture of AN-B and MC-LR exhibited the strongest toxic effects, providing the first direct evidence of synergistic toxic effects of cyanopeptides on physiological parameters in Daphnia magna.