Journal
TISSUE & CELL
Volume 72, Issue -, Pages -Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2021.101573
Keywords
Intestinal microvascular endothelial cell; Characterization; Gut-vascular barrier; Endothelial cell line
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Funding
- National Natural Science Foundation of China [31972727, 31902315]
- ZAFU Research and Development Fund grant [203402005001]
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The newly established rat intestinal microvascular endothelial cell line RIMVEC-11, characterized by endothelial features, maintained fundamental physiological function, and possessed potential for capillary formation and cell migration.
Intestinal microvascular endothelial cell (IMVEC) is a fundamental and essential component of gut-vascular barrier which is closely associated with intestinal disorders However, there is still a lack of established intestinal microvascular endothelial cell line. In the present study, a newly established rat intestinal microvascular endothelial cell line termed RIMVEC-11 was described and characterized which has been stably cultured for more than 90 passages so far. RIMVEC-11 was characterized by endothelial features with the cobblestone morphology under light microscopy, the Weibel-Palade body and rich vesicles in the cytoplasm on the ultrastructural level, and positive endothelial specific markers CD31 and von Willebrand factor by immunocyto-chemistry analysis. Meanwhile, RIMVEC-11 maintained the fundamental physiological function of the microvascular endothelial cells. Tube formation assay confirmed that RIMVEC-11 retained the potential for capillaries formation. Scratch assay confirmed the endothelial cell migration potential of RIMVEC-11. Thus, a novel IMVEC cell line RIMVEC-11 was established, which could be used as a promising model for the gut-vascular barrier research.
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