Journal
TISSUE & CELL
Volume 73, Issue -, Pages -Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2021.101615
Keywords
Cortisol; Satellite cell; Fibroblast; Co-culture; Cell viability; Myogenesis
Categories
Funding
- Next-Generation Bio Green 21 Program [PJ01316702]
- Rural Development Administration, Republic of Korea
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The study showed that exogenous cortisol reduced cell viability and had an impact on cell nucleus and cell cycle in primary cultured satellite cells and fibroblasts of porcine gluteal muscles.
Cortisol is a ubiquitously expressed stress hormone. In this study, we investigated the effects of exogenous cortisol on porcine gluteal muscles primary cultured satellite cells and fibroblasts. Satellite cells and fibroblasts were mono-or co-cultured, and cells in each type of culture were categorized into the control and cortisol-treated (treatment) groups. We selected 28 mu mol mL-1 cortisol for treatment based on their efficacy. Cortisol treatment reduced viability of monocultured satellite cells and fibroblasts. In both monocultured and co-cultured cells, the nucleus in the treatment group was damaged than that control group. Moreover, the total cell cycle duration was shorter in the treatment group than the control group. PAX-7 expression was upregulated in the control group of co-cultured satellite cells and fibroblasts than those remaining groups. Moreover, MyoD expression was downregulated in the cortisol treated group of both mono-and co-cultured satellite cells compared with that in the control group. In co-cultured fibroblasts, MyoD and MyoG expression was upregulated than those remaining groups. The Cyto-C expression was upregulated in the treatment group compared to the control mono-and cocultured both cells. These results suggest that the selected experimental dose of cortisol reduced cell viability and myogenesis-related gene expression in the monoculture compared to that in the co-culture of satellite cells and fibroblasts.
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