Journal
TALANTA
Volume 233, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2021.122601
Keywords
Fluorescent probe; Near-infrared; Dual-response; Fe2+ and H+; BODIPY
Categories
Funding
- National Natural Science Foundation of China [21907054]
- Fundamental Research Funds from Nankai University [ZB19100136]
- Fundamental Research Funds for the Central Universities, Nankai University [2122018165]
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BODIPYFe is a dual-responsive near-infrared fluorescent probe that can rapidly respond to ferrous ions with high sensitivity. It can sensitively and selectively detect ferrous ions and H+ in the lysosomes of living cells simultaneously, demonstrating great potential for simultaneous imaging of Fe2+ and H+ in biological systems.
Ferrous ion (Fe2+) plays an essential role in many physiological and pathological processes, and its cellular metabolism is closely related to acidic pH. However, the lack of multifunctional Fe2+ probes has hindered the further study of Fe2+ in vivo. Herein, we report a dual-responsive near-infrared (NIR) fluorescent probe BODIPYFe for the simultaneous of Fe2+ and H+ in vivo by harnessing the N-oxide strategy and photoinduced electron transfer (PeT) mechanism. BODIPY-Fe exhibited NIR fluorescence at 671 nm, rapid response to Fe2+ within 90 s, and high sensitivity of low LOD of 292 nM towards Fe2+. Moreover, BODIPY-Fe could sensitively and selectively detect Fe2+ and H+ in the lysosomes of living cells simultaneously. Notably, BODIPY-Fe was able to non-invasively visualize Fe2+ and H+ in vivo, showing ON-OFF-ON NIR fluorescence signal changes. This work demonstrates that BODIPY-Fe has great potential to promote the simultaneous imaging of Fe2+ and H+ in biological systems.
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