Journal
TALANTA
Volume 236, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2021.122831
Keywords
COF; Boronic acid; Post-synthesis modification; Glycopeptides; MALDI-TOF MS; Nano-LC-MS; MS
Categories
Funding
- National Natural Science Foundation of China [21927805]
- National Key R&D Program of China [2016YFF0102900]
- K. C. Wong Magna Fund in Ningbo University
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This study successfully prepared a novel COF material with high sensitivity and selectivity through post-synthesis modification, which showed significant enrichment effect on glycopeptides and is expected to be applied in glycoproteomics research.
In this study, a novel type of covalent organic framework (COF) material rich in boronic acid sites was prepared through post-synthesis modification (TbBD@PEI@Au@4-MPBA). The surface of COF material had abundant carboxylic acid groups, which could bind a large amount of polyethyleneimine (PEI) through electrostatic interaction. At the same time, the amino groups on the PEI can be grafted with Au nanoparticles (Au NPs) in situ, and then 4-mercaptophenylboronic acid (4-MPBA) was modified by the reaction of Au and sulfhydryl groups. The massive grafting of boronic acid groups made the material's enrichment effect on glycopeptides expected. The results of experiments indicated that the composite material has high sensitivity (5 amol mu L-1) and selectivity (1:1000). In addition, the material has outstanding stability and reusability, with a load capacity of about 100 mg g-1 and a recovery of 99.3 +/- 2.2%. What's more, after enriched by TbBD@PEI@Au@4-MPBA, 56 endogenous glycopeptides from fresh human saliva were detected by MALDI-TOF MS, 56 unique glycopeptides corresponding to 31 glycoproteins from human saliva and 513 unique glycopeptides corresponding to 208 glycoproteins from serum of throat cancer patient were detected by nano-LC-MS/MS, respectively, which was expected to be applied to glycoproteomics research.
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