Journal
TALANTA
Volume 237, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2021.122970
Keywords
ZnO; Bio-TFT; Escherichia coli EPEC; Real time detection
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The fabrication of biosensors for eco-friendly technologies has various future applications. Label-free strategies, recyclable materials, and low-temperature processing are important considerations. In this study, Zinc oxide Thin-film Transistors were used for real-time enteropathogenic Escherichia coli detection, with Fourier Transform Infrared Spectroscopy verifying the characteristic absorption peaks during the bio-TFT assembly process.
The fabrication of biosensors has different future applications mainly from the perspective of eco-friendly technologies. Label-free strategies, recyclable materials and low-temperature processing are parameters to consider for the development of a new generation of biosensor devices. In this work, Zinc oxide (ZnO) Thin-film Transistors (TFTs) using recyclable plastic substrates were used for real-time enteropathogenic Escherichia coli detection as an approach for biosensing (bio-TFTs). Fourier Transform Infrared Spectroscopy was used to verify the characteristic absorption peaks at the different steps of the bio-TFT assembly process. The bio-TFTs are ready to observe the bacterial detection by electrical characterization. Finally, detection was validated by a coupled strategy that fuses the genomic DNA extraction from bacteria attached in situ over bio-TFTs surface and, the development of the Polymerase Chain Reaction to amplify specific genes from enteropathogenic Escherichia coli.
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