Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.

Automated summary

Skin punch biopsy from a healthy 51-year-old Caucasian male was used to derive dermal fibroblasts, which were reprogrammed into hiPSC lines using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4, and c-MYC. The resulting iPSC lines, capable of expressing pluripotent markers and differentiating into cells of endodermal, mesodermal, and ectodermal origin, can serve as controls for human disease modeling and drug screening when combined with genome-editing and 3D systems.