Journal
STEM CELL RESEARCH
Volume 57, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scr.2021.102574
Keywords
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Funding
- Ministry of Health of the Czech Republic [NV18-08-00412]
- Faculty of Medicine, Masaryk University [MUNI/A/1689/2020]
- European Regional Development Fund-Project MAGNET [CZ.02.1.01/0.0/0.0/15_003/0000492]
- Brno City Municipality
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The research presented the generation of two sex-discordant sibling hESC lines through mechanical biopsy under xeno-and feeder-free conditions. This approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno-and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
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