Labeling of liver cells with CdSe/ZnS quantum dot-based fluorescence probe below freezing point

In this paper, CdSe/ZnS quantum dots (QDs) with particle size of 5.5 -9.3 nm were synthesized, and the fluorescence emission ranged from 545 -616 nm. When the volume fraction of ethanol was 30%, the water-soluble QD dispersion system remained liquid under-20 degrees C freezing conditions, the fluorescence intensity increased with a decrease in temperature, and the quantum yield reached 79% at-20 degrees C. The endothelial cell adhesion molecule CD31 antibody (anti-CD31) was used as the primary antibody, QDs were coupled with IgG as the secondary antibody (QD-Ab), and effective labeling of hepatic sinusoid endothelial cells was achieved at-20 degrees C. Fluorescence imaging and flow cytometry analysis showed that the labeling efficiency was as high as 97%, indicating that QDs have an important application prospect in microscopic section tomography of the liver. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

In this study, CdSe/ZnS quantum dots with a particle size of 5.5-9.3 nm were successfully synthesized, showing fluorescence emission ranging from 545-616 nm. The water-soluble QD dispersion system remained liquid under freezing conditions at -20 degrees C with a fluorescence intensity increase and a quantum yield of 79% at -20 degrees C. Utilizing QDs, effective labeling of hepatic sinusoid endothelial cells was achieved with a labeling efficiency as high as 97%, indicating promising applications in microscopic liver section tomography.