A sensitive biosensor for glucose determination based on the unique catalytic chemiluminescence of sodium molybdate

Chemiluminescent (CL) reaction between hydrogen peroxide (H2O2) and luminol was dramatically enhanced by sodium molybdate (Na2MoO4) for 284-fold. CL mechanism investigation indicated that Na2MoO4 increased the production of hydroxyl radical ((OH)-O-center dot) and superoxide anion (O-center dot(2)) in the H2O2 luminol system, which could attribute to the enhanced-CL intensity and gave us new insights into the CL-enhanced property of Na2MoO4. The CL intensity of Na2MoO4-H2O2-luminol system increased with the concentration of H2O2, based on which, a convenient and sensitive CL determination method could be developed for H2O2 in the concentration ranging from 0.5 to 60 mu mol/L, with a detection limit of 0.25 mu mol/L. Combining with glucose oxidase, the Na2MoO4-H2O2-luminol system could also be applied for glucose detection. Glucose in human serum has been successfully detected with satisfied recoveries in the range of 96.7 % to 105.4 %. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The study demonstrates that sodium molybdate significantly enhances the chemiluminescent reaction between hydrogen peroxide and luminol, providing a convenient and sensitive method for hydrogen peroxide determination, as well as potential application for glucose detection when combined with glucose oxidase. This finding suggests promising applications of sodium molybdate in analytical chemistry.