Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 264, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.120257
Keywords
Peptide; Fluorescent probe; Cu2+; CN-; Cells imaging
Categories
Funding
- Application Foundation Project of Science and Technology Department of Sichuan Province [2021YJ0409, 2021YJ0323]
- Fundamental Research Funds of China West Normal University [CXTD2020-1, 416628/18B018]
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The novel fluorescent probe DSD exhibits high sensitivity towards Cu2+ and CN with high selectivity and rapid recognition. It features a large Stokes shift, perfect water solubility, and the ability to be reversibly cycled at least 10 times.
A novel fluorescent probe (DSD) was reasonably designed and synthesized with dansyl-labeled dipeptide (Dan-Ser-Asp-NH2). DSD featured remarkably large Stokes shift (230 nm) and perfect water solubility, and exhibited high selectivity and rapid recognition toward Cu2+ via fluorescence quenching. The detection limit of DSD for Cu2+ was 2.4 nM, indicated that DSD has excellent sensitivity. In addition, the stoichiometry between DSD and Cu2+ were detected as 1:1 by fluorescence titration, Job's plot and ESI-HRMS data. As designed, DSD-Cu2+ system was able to sequentially detect CN according to the displacement approach with fluorescence off-on response, and the detection limit for CN was calculated to be 41.9 nM. Specifically, the response time of DSD with Cu2+ and CN was less than 40 s, which rendered it suitable for real time detection in actual water samples. In addition, with the alternate addition of Cu2+ and CN , the reversible cycles could be repeated for at least 10 times, indicated that DSD was a promising reversibility probe. DSD showed low toxicity and good biocompatibility, and was successfully applied to detect Cu2+ and CN in living cells. (C) 2021 Elsevier B.V. All rights reserved.
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