Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 259, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.119826
Keywords
Cysteine; Fluorescence; Imaging; Sensor; Detection; Biothiol
Categories
Funding
- National Natural Science Foundation of China [81801750]
- Natural Science Foundation of Henan Province [212300410222]
- Key Scientific Research Foundation of the Higher Education Institutions of Henan Province, China [21B350002, 19A150006]
- Key Project of Science and Technology of Henan Province [182102310323]
- Excellent Youth Science Foundation of Henan Province [202300410310]
Ask authors/readers for more resources
A red-emitting probe TMN-NCS based on ITC structure was proposed in this paper, which has high selectivity and sensitivity to Cys and shows great potential for biological applications with low toxicity, low detection limit, and good cell permeability.
Cysteine (Cys) is an effective biomarker in life systems and is closely related to a variety of diseases, so developing a specific and efficient detection method for Cys is of great significance. To date, extensive work has been undertaken toward this goal. However, the differentiation of Cys from other biothiols still represents a challenge from an experimental point of view. Toward this end, a selective and sensitive red-emitting probe (TMN-NCS) with an isothiocyanate (ITC)-based structure was proposed in this paper. A large Stokes shift (210 nm) was observed upon addition of Cys to a solution of TMN-NCS. In addition, TMN-NCS showed low toxicity, a low detection limit (120 nM), and excellent cell permeability. The results suggested that TMN-NCS holds great promise for biological applications. (c) 2021 Elsevier B.V. All rights reserved.
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