Journal
SMALL
Volume 18, Issue 13, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202104758
Keywords
3D printing; bioactive glass scaffolds; cell-bioengineering; extracellular matrix; injectable hydrogels; self-assembled matrix
Categories
Funding
- Bundesministerium fur Bildung und Forschung (BMBF) [BMBF: 03Z22E511]
- Projekt DEAL
Ask authors/readers for more resources
Stem cell bioengineering and therapy require different model systems and materials. A biomatrix system optimal for 2D hMSC culture and osteogenesis has been identified through screening biomaterials synthesized through a self-assembling mechanism. This biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.
Stem cell bioengineering and therapy require different model systems and materials in different stages of development. If a chemically defined biomatrix system can fulfill most tasks, it can minimize the discrepancy among various setups. By screening biomaterials synthesized through a coacervation-mediated self-assembling mechanism, a biomatrix system optimal for 2D human mesenchymal stromal cell (hMSC) culture and osteogenesis is identified. Its utility for hMSC bioengineering is further demonstrated in coating porous bioactive glass scaffolds and nanoparticle synthesis for esiRNA delivery to knock down the SOX-9 gene with high delivery efficiency. The self-assembled injectable system is further utilized for 3D cell culture, segregated co-culture of hMSC with human umbilical vein endothelial cells (HUVEC) as an angiogenesis model, and 3D bioprinting. Most interestingly, the coating of bioactive glass with the self-assembled biomatrix not only supports the proliferation and osteogenesis of hMSC in the 3D scaffold but also induces the amorphous bioactive glass (BG) scaffold surface to form new apatite crystals resembling bone-shaped plate structures. Thus, the self-assembled biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available