Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 353, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.131016
Keywords
Turn-on sensor; Tetraphenylethylene derivative; Heparin-Protamine interaction; Heparin sensor, Protamine sensor, Heparinase sensor
Funding
- Bhabha Atomic Research Centre, Mumbai, India
- Amity Institute of Biotechnology, Amity University, Mumbai
- CSIR-UGC
- UGC-FRP
- Lady Tata Memorial Trust
Ask authors/readers for more resources
This study successfully utilized tetrapyridinium-tetraphenylethylene (TPy-TPE) as a fluorescent probe for detecting heparin, showing improved sensitivity and performance. TPy-TPE also detected the antidote and degrading enzyme of heparin.
Designing efficient fluorescent probes with Aggregation Induced Emission (AIE) feature, especially for sensing biologically important analytes, is an intensive area of investigation. Heparin is a well-known blood anticoagulant, and is routinely used as a very important medication in clinical setups. In this contribution, we have utilized a tetraphenylethylene derived AIEgen, named tetrapyridinium-tetraphenylethylene (TPy-TPE), for sensing Heparin. TPy-TPE carries four positive charges and forms aggregates in presence of negatively charged Heparin to generate highly emissive aggregates of TPy-TPE. Most importantly, the tetracationic nature of TPyTPE provides a stronger binding affinity towards Heparin compared to the previously reported monocationic or dicationic fluorophores. The improved interaction between Heparin and TPy-TPE has proved to be advantageous in terms of improved sensitivity (lower LODs in aqueous and serum samples), higher fluorescence enhancement and better performance in complex matrices. While TPy-TPE is utilized for Heparin determination, its sole U.S. Food and Drug Administration (FDA) authorized antidote, Protamine, and Heparinase, which specifically degrades Heparin to produce clinically important Low Molecular Weight Heparin (LMWH) have also been detected through TPy-TPE molecule. Overall, the photophysical investigation of TPy-TPE for the detection of Heparin is a successful attempt and is expected to enhance Heparin related biosensing research in the biomedical fields.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available