Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 353, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.131032
Keywords
Metal-organic framework; self-calibrating nanoprobe; turn-on fluorescence; hypochlorite; bioimaging
Funding
- National Natural Science Foundation of China [82061148012, 82027806, 91753106, 21974019, 92061121]
- National Key Research and Development Program of China [2017YFA0205300]
- Primary Research & Development Plan of Jiangsu Province [BE2019716]
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The study developed a method using UiO-66-NH2 as a nanoscale probe to detect hypochlorite, aiming to explore the critical role of ROS in signaling and pathological processes. Additionally, the research also demonstrated that other amino-group functionalized MOF-based sensors exhibited similar fluorescence response towards ClO-, providing a new insight for developing highly specific and efficient probes.
Developing fluorescent probes to detect reactive oxygen species (ROS) is imperative to understanding the vital roles of ROS in various signaling and pathological processes. In this work, UiO-66-NH2 could be readily utilized as self-calibrating nanoprobe for selective detection and bioimaging of hypochlorite. Under the excitation of 400 nm, UiO-66-NH2 featured an emission at 432 nm and a new emission at 533 nm appeared after the addition of ClO-. Besides, the emission intensity of 533 nm increased with the incremental amount of ClO-. By using the ratio I-533 nm/I-432 nm as detection signal, UiO-66-NH2 could discriminate ClO- with high performance. The linear working range towards ClO- is 0.1-150 mu M and the detection limit is 63.8 nM. In contrast to most other ratiometric sensors fabricated by physical entrapment methods, UiO-66-NH2 has the obvious advantage of effectively avoiding the worry of leakage of fluorophores from the nanomaterial matrix of sensors leading to erroneous measurements. Other amino-group functionalized MOF-based sensors such as MIL-53-NH2(Al) and MIL-101NH(2)(Fe) exhibited similar fluorescence response toward ClO- as UiO-66-NH2. This work raises the possibility to develop highly specific and efficient probe for ClO- detection in vivo or in living cells and presents a new insight into ratiometric sensors.
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