Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 346, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130581
Keywords
Electrochemiluminescence; Aptamer; Fe3O4@Cu@Cu2O nanocomposite; [Ru(bpy)(2)dppz](2+); Adenosine triphosphate (ATP)
Funding
- National Key Scientific Instrument and Equipment Development Project of China [21627809]
- National Natural Science Foundation of China [21777056]
- Science and Technology Development Plan Project of Shandong Province [2018GSF118209]
- Special Foundation for Taishan Scholar Professorship of Shandong Province, Jinan Scientific Research Leader Workshop Project [2018GXRC024]
- Shandong Provincial Natural Science Foundation [ZR2020QB097]
- Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province
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A novel and facile ECL platform was developed to detect ATP based on target-triggered DNA conformation change and emitter release, achieving high sensitivity and excellent specificity. This platform has the potential to be a versatile tool for biomolecule detection in clinical diagnosis and bioanalysis.
For most of the sensitive electrochemiluminescence (ECL) aptasensors, the probe molecules often need complex chemical modification to the aptamer or target. Herein, a novel and facile ECL platform was fabricated to detect adenosine triphosphate (ATP), based on the target-triggered DNA conformation change and emitter release. Concretely, the luminophore, [Ru(bpy)(2)dppz](2+), was anchored on single strand DNA owing to its extended aromatic structure and the electrostatic attraction. Benefiting from this model, sophisticated chemical labeling was avoided. Additionally, mesopomus Fe3O4@Cu@Cu2O nanocomposite was synthesized as the substrate to load plenty of ATP aptamers, and for the first time, it was used to expedite the oxidation of co-reactant TEA, achieving abundant cation radicals (TEA(center dot+)) and enhanced ECL signal. As a function of relative ATP concentration, changes in ECL intensity, was further studied, where a favorable linear range from 0.5 nmol/L to 2500 nmol/L was obtained with a low detection limit of 0.17 nmol/L. On account of the abovementioned points, this proposed ECL aptasensor exhibited high sensitivity and excellent specificity. These results demonstrate that the present platform may open a window to develop versatile implement for biomolecules detection in clinical diagnosis and bioanalysis.
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