Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 344, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130242
Keywords
Colorimetric detection; SARS-CoV-2; Human enteric pathogens; 3D printed integrated microfluidic chip; Smartphone-based detection platform
Funding
- USDA/AFRI [2016-08811]
- NIH [R01EB023607, R01CA214072]
- UConn COVID-19 Rapid Start Funding (COVID-RSF)
- School of Engineering GE Fellowship at the University of Connecticut
- Department of Civil and Environmental Engineering Undergraduate Research Initiative at the University of Connecticut
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The study developed a sensitive, multiplexed, colorimetric detection method for pathogen detection in wastewater samples, allowing for early warning of disease outbreaks. This method integrated on-chip nucleic acid extraction, two-stage isothermal amplification, and colorimetric detection on a 3D printed microfluidic chip, enabling real-time analysis without complex equipment.
Severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic has become a global public health emergency. The detection of SARS-CoV-2 and human enteric pathogens in wastewater can provide an early warning of disease outbreak. Herein, a sensitive, multiplexed, colorimetric detection (termed SMCD) method was established for pathogen detection in wastewater samples. The SMCD method integrated on-chip nucleic acid extraction, two-stage isothermal amplification, and colorimetric detection on a 3D printed microfluidic chip. The colorimetric signal during nucleic acid amplification was recorded in real-time and analyzed by a programmed smartphone without the need for complicated equipment. By combining two-stage isothermal amplification assay into the integrated microfluidic platform, we detected SARS-CoV-2 and human enteric pathogens with sensitivities of 100 genome equivalent (GE)/mL and 500 colony-forming units (CFU)/mL, respectively, in wastewater within one hour. Additionally, we realized smart, connected, on-site detection with a reporting framework embedded in a portable detection platform, which exhibited potential for rapid spatiotemporal epidemiologic data collection regarding the environmental dynamics, transmission, and persistence of infectious diseases.
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