Ultrasensitive pathogenic bacteria detection by a smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay

Article Biochemistry & Molecular Biology

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

Parinaz Fozouni et al.

Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.

CELL (2021)

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Article Chemistry, Analytical

Exploring the Trans-Cleavage Activity of CRISPR/Cas12a on Gold Nanoparticles for Stable and Sensitive Biosensing

Xiaoyi Fu et al.

Summary: Taking advantage of the trans-cleavage activity of CRISPR/Cas12a on gold nanoparticles, a new spherical nucleic acid (SNA) reporter has been developed for stable and sensitive CRISPR-Dx biosensing. Different trans-cleavage activities of Cas12a systems were discovered on a nanoparticle surface, with LbCas12a highly dependent on DNA strand density and length. The SNA reporter exhibited improved stability and high sensitivity, showing potential for future research in biology science and medical diagnoses.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria

Anzhi Sheng et al.

Summary: In this study, it was found that MXene can efficiently adsorb single-stranded DNA and be coupled with CRISPR-Cas12a for sensitive detection of LPS and bacteria. Through carefully designed aptamers, the trans-cleavage activity of CRISPR-Cas12a is activated, allowing for selective and sensitive quantification of targets in different samples. This method provides a new direction for the exploration of universal analytical methods based on MXene coupled with CRISPR-Cas12a.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

Light-up RNA aptamer signaling-CRISPR-Cas13a-based mix-and-read assays for profiling viable pathogenic bacteria

Ting Zhang et al.

Summary: This study developed a new detection system that can accurately distinguish viable pathogenic bacteria without the need for reverse transcription, nucleic acid amplification, or labeled substrates. By utilizing CRISPR-Cas13a and a light-up RNA aptamer, it enables rapid detection and precise quantification of viable pathogens.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection

Tao Li et al.

Summary: By utilizing G-triplex (G3) as a reporter gene, G-CRISPR achieves a 20-fold improvement in analytical sensitivity, enabling detection of unamplified and amplified DNA as low as 50 pmol and 0.1 amol (one copy/reaction) respectively. The results demonstrate high specificity and sensitivity, and suggest that G-CRISPR could serve as a rapid, sensitive, and reliable biosensor in biomedical diagnostics.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Chemistry, Analytical

Recent advances on G-quadruplex for biosensing, bioimaging and cancer therapy

Jiaqi Xu et al.

Summary: G-quadruplex is a three-dimensional secondary structure of nucleic acids formed by Hoogsteen hydrogen pairing of four guanines, with various topologies applicable in biosensing and bioimaging. By incorporating fluorescence dyes or forming HRP-mimicking DNAzyme, G-quadruplex-based biosensors enable sensitive and selective detection of a wide range of molecules, thereby contributing to cancer therapy.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

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Review Engineering, Biomedical

CRISPR-based diagnostics

Michael M. Kaminski et al.

Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.

NATURE BIOMEDICAL ENGINEERING (2021)

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Article Multidisciplinary Sciences

A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics

Kai Shi et al.

Summary: CRISPR-Cas-only amplification network (CONAN) utilizes a Cas12a-driven reaction network to construct a positive feedback circuit, enabling isothermally amplified detection of genomic DNA with attomolar sensitivity, showcasing its potential as a powerful tool for disease diagnostics.

SCIENCE ADVANCES (2021)

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Article Multidisciplinary Sciences