Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 344, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130166
Keywords
Indoleamine-2; 3-dioxygenase (IDO); Tryptophan (Trp); Kynurenine (Kyn) pathway (KP); Surface-enhanced Raman scattering (SERS); Biologically Raman-silent region; Cancer
Funding
- Fujian Provincial Department of Science and Technology guiding Project [2020Y0019]
- Youth and middle-aged backbone talents training project of Fujian Health Commission [2020GGA003]
- Industry-university Cooperation Project of Fujian Provincial Department of Science and Technology [2020N5006]
- National Natural Science Foundation of China [61975031, 11874006]
- Natural Science Foundation of Fujian Province of China [2016J01292, 2018J01786]
- Achievement Transformation Project of Fuzhou Science and Technology Bureau [2020GX20]
- Fushimei Agricultural and rural maker space (Minke xing (2019)) [2]
- Innovative Research Team in Science and Technology in Fujian Province University
Ask authors/readers for more resources
A SERS sensor triggered by enzyme was developed for detecting IDO to determine the presence of colon cancer, with a low limit of detection.
As a hitman of human health, colon cancer has become the third most common cancer in the world in the recent years. Indoleamine-2,3-dioxygenase (IDO) was found to be closely related to colon cancer. Herein, an on-off SERS sensor triggered by enzyme was developed for detecting IDO for presence or absence of colon cancer based on SERS, which composed of Au@MBN@Ag NPs functionalized by aptamer, Tryptophan (Trp) and carboxylated magnetic beads (MNP-COOH). Thus, Au@MBN@Ag NPs were separate from MNP-COOH with the assistance of IDO that catalyzed tryptophan to Kyn, leading to the reduction of SERS intensity of MBN as the increase of the concentration of IDO. Through the method performed within 60 min, the volue of the limit of detection (LOD) is lower to 0.278 ng/mL for IDO ranged from 3.125 ng/mL to 625 ng/mL can be achieved. Additionally, the results of controlled experiment demonstrated that the enzyme-triggered switchable nanoprobes have high specificity for the detection of IDO. Meanwhile, the feasibility of the proposed method in macrophages and clinical serum were also confirmed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
