Journal
SENSORS
Volume 21, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/s21216945
Keywords
real-time PCR; multiplex; filter wheel; PCR chip; point of care; open platform camera
Funding
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HW20C2053]
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This paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. The system utilizes an open platform camera with embedded lens and a compact filter wheel, which performs comparably to photodiode systems in fluorescence detection.
The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45 & DEG; angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 mu m. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.
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