Journal
SENSORS
Volume 22, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/s22031198
Keywords
Arabidopsis thaliana; biosensor; fluorescent; transporter
Funding
- Academia Sinica, Taiwan, and Ministry of Science and Technology, Taiwan [MOST 109-2311-B-001 -021]
- [AS-CFII-108-116]
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In this study, an uncharacterized member of the NPF family in Arabidopsis, NPF1.3, was converted into a nitrate-specific transporter activity biosensor, demonstrating its important role in nitrate transport in Arabidopsis.
The NRT1/PTR FAMILY (NPF) in Arabidopsis (Arabidopsis thaliana) plays a major role as a nitrate transporter. The first nitrate transporter activity biosensor NiTrac1 converted the dual-affinity nitrate transceptor NPF6.3 into fluorescence activity sensors. To test whether this approach is transferable to other members of this family, screening for genetically encoded fluorescence transport activity sensor was performed with the member of the NPF family in Arabidopsis. In this study, NPF1.3, an uncharacterized member of NPF in Arabidopsis, was converted into a transporter activity biosensor NiTrac-NPF1.3 that responds specifically to nitrate. The emission ratio change of NiTrac-NPF1.3 triggered by the addition of nitrate reveals the important function of NPF1.3 in nitrate transport in Arabidopsis. A functional analysis of Xenopus laevis oocytes confirmed that NPF1.3 plays a role as a nitrate transporter. This new technology is applicable in plant and medical research.
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