Journal
INTEGRATIVE BIOLOGY
Volume 8, Issue 4, Pages 564-570Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ib00301f
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Funding
- DFG Research Training Group GRK2062 Molecular Principles of Synthetic Biology
- DFG Cluster of Excellence NIM
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Position-dependent gene expression in gradients of morphogens is one of the key processes involved in cellular differentiation during development. Here, we study a simple artificial differentiation process, which is based on the diffusion of genetic inducers within one-dimensional arrangements of 50 mm large water-in-oil droplets. The droplets are filled with either bacteria or cell-free gene expression systems, both equipped with genetic constructs that produce inducers or respond to them via expression of a fluorescent protein. We quantitatively study the coupled diffusion-gene expression process and demonstrate that gene expression can be made position-dependent both within bacteria-containing and cell-free droplets. By generating diffusing quorum sensing signals in situ, we also establish communication between artificial cell-free sender cells and bacterial receivers, and vice versa.
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