Journal
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
Volume 126, Issue -, Pages 117-124Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.semcdb.2021.11.004
Keywords
Intrabody; Brain; Neuron; ScFv; Nanobody; FingR; Recombinant; Plasmid; Live cell imaging
Categories
Funding
- National In-stitutes of Health [U24NS109113]
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Intrabodies are recombinant antibodies designed for expression within neurons, allowing for precise targeting of specific molecules and subcellular domains to study and manipulate neuronal function with high precision.
Basic neuroscience research employs numerous forms of antibodies as key reagents in diverse applications. While the predominant use of antibodies is as immunolabeling reagents, neuroscientists are making increased use of intracellular antibodies or intrabodies. Intrabodies are recombinant antibodies genetically encoded for expression within neurons. These can be used to target various cargo (fluorescent proteins, reporters, enzymes, etc.) to specific molecules and subcellular domains to report on and manipulate neuronal function with high precision. Intrabodies have the advantages inherent in all genetically encoded recombinant antibodies but represent a distinct subclass in that their structure allows for their expression and function within cells. The high precision afforded by the ability to direct their expression to specific cell types, and the selective binding of intrabodies to targets within these allows intrabodies to offer unique advantages for neuroscience research, given the tremendous molecular, cellular and morphological complexity of brain neurons. Intrabodies expressed within neurons have been used for a variety of purposes in basic neuroscience research. Here I provide a general background to intrabodies and their development, and examples of their emerging utility as valuable basic neuroscience research tools.
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