Journal
SCIENCE SIGNALING
Volume 15, Issue 720, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.abd9099
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Categories
Funding
- Cancer Research UK [C596/A26855, A29799]
- Francis Crick Institute - Cancer Research UK [FC001557]
- United Kingdom Medical Research Council [FC001557]
- Wellcome Trust [FC001557, WT107492Z]
- CRUK Beatson Institute - Cancer Research UK [C596/A17196, A31287]
- Cancer Research UK Beatson Institute [C596/A23983]
- Breast Cancer Now [2019DecPR1424]
- CRUK HUNTER Accelerator Award [A26813]
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Genetically encoded probes are commonly used to visualize cellular processes, but traditional fluorescent protein tags are suboptimal for in vivo studies due to poor tissue penetration and high background signal. A new knock-in reporter mouse model linking near-infrared fluorescent protein expression to a synthetic p53-responsive promoter enables noninvasive, longitudinal analysis of p53 activity in vivo. This model has provided insights into the timing and localization of p53 activation in response to various stimuli, potentially advancing our understanding of physiological and pathophysiological p53 responses.
Genetically encoded probes are widely used to visualize cellular processes in vitro and in vivo. Although effective in cultured cells, fluorescent protein tags and reporters are suboptimal in vivo because of poor tissue penetration and high background signal. Luciferase reporters offer improved signal-to-noise ratios but require injections of luciferin that can lead to variable responses and that limit the number and timing of data points that can be gathered. Such issues in studying the critical transcription factor p53 have limited insight on its activity in vivo during development and tissue injury responses. Here, by linking the expression of the near-infrared fluorescent protein iRFP713 to a synthetic p53-responsive promoter, we generated a knock-in reporter mouse that enabled noninvasive, longitudinal analysis of p53 activity in vivo in response to various stimuli. In the developing embryo, this model revealed the timing and localization of p53 activation. In adult mice, the model monitored p53 activation in response to irradiation and paracetamol- or CCl4-induced liver regeneration. After irradiation, we observed potent and sustained activation of p53 in the liver, which limited the production of reactive oxygen species (ROS) and promoted DNA damage resolution. We propose that this new reporter may be used to further advance our understanding of various physiological and pathophysiological p53 responses.
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