Anti-oxidant mechanisms of Chlorella pyrenoidosa under acute GenX exposure

GenX, a substitute for perfluorooctanoic acid, has been widely detected in surface water. Due to its bioaccumulation, toxicity and persistence, GenX can cause adverse effects such as oxidative damage on aquatic organisms. To investigate the toxicity of GenX and the anti-oxidant mechanism of algae under acute exposure, the growth, photosynthetic activity and gene expression of Chlorella pyrenoidosa (C. pyrenoidosa) were tested. Results showed that the growth of C. pyrenoidosa was inhibited under acute GenX exposure. The toxicity of GenX increased with time and concentration but was lower than that of the traditional perfluoroalkyl and polyfluoroalkyl substances (PFASs). Furthermore, with the increase of GenX concentration, the production of reactive oxygen species increased, while the level of the anti-oxidant enzyme first increased and then decreased. Changes in photosynthetic parameters also indicated that the photosynthetic system of C. pyrenoidosa was negatively affected by GenX exposure. Transcription analysis revealed that the up-regulation of genes related to the glutathione-ascorbate cycle and photosynthesis is a positive strategy to cope with the oxidative stress caused by acute GenX exposure. Our findings provide new insights into the interactions between emerging PFASs and aquatic organisms at the molecular level. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

GenX, a substitute for perfluorooctanoic acid, has been widely detected in surface water and has potential ecological hazards. Research shows that acute exposure to GenX inhibits the growth of Chlorella pyrenoidosa and damages its photosynthetic system, but the up-regulation of related genes provides an antioxidant mechanism. This study provides new insights into the antioxidant capacity to cope with the oxidative stress caused by acute GenX exposure.